SELECTED PUBLICATIONS


Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, Parrington J, Galione A. (2015) Expression of Ca²⁺-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells. EMBO J. pii: e201490009 [Epub ahead of print]


Lear PV, González-Touceda D, Porteiro Couto B, Viaño P, Guymer V, Remzova E, Tunn R, Chalasani A, García-Caballero T, Hargreaves IP, Tynan PW, Christian HC, Nogueiras R, Parrington J, Diéguez C. (2015) Absence of intracellular ion channels TPC1 and TPC2 leads to mature-onset obesity in male mice, due to impaired lipid availability for thermogenesis in brown adipose tissue. Endocrinology 156(3): 975-86


Lin PH, Duann P, Komazaki S, Park KH, Li H, Sun M, Sermersheim M, Gumpper K, Parrington J, Galione A, Evans AM, Zhu MX, Ma J. (2015) Lysosomal two-pore channel subtype 2 (TPC2) regulates skeletal muscle autophagic signaling. J Biol Chem 290(6): 3377-89

Favia A, Desideri M, Gambara G, D'Alessio A, Ruas M, Esposito B, Del Bufalo D, Parrington J, Ziparo E, Palombi F, Galione A, Filippini A. (2014) VEGF-induced neoangiogenesis is mediated by NAADP and two-pore channel-2-dependent Ca²⁺ signaling. Proc Natl Acad Sci U S A 111: E4706-15

Ruas M, Chuang KT, Davis LC, Al-Douri A, Tynan PW, Tunn R, Teboul L, Galione A, Parrington J. (2014) TPC1 has two variant isoforms, and their removal has different effects on endo-lysosomal functions compared to loss of TPC2. Mol Cell Biol 34: 3981-92

Parrington J, Tunn R. (2014) Ca²⁺ signals, NAADP and two-pore channels: role in cellular differentiation. Acta Physiol (Oxf) 211: 285-96

Kashir J, Heindryckx B, Jones C, De Sutter P, Parrington J, Coward K. (2012) A sperm's tail: the need to consider temporal aspects of specific physiological mechanisms during and following gamete fusion. Hum Reprod 27: 625-6


Rietdorf K, Funnell TM, Ruas M, Heinemann J, Parrington J, Galione A. (2011) Two-pore channels form homo- and heterodimers. J Biol Chem 286: 37058-62

Aghajanpour S, Ghaedi K, Salamian A, Deemeh MR, Tavalaee M, Moshtaghian J, Parrington J, Nasr-Esfahani MH. (2011) Quantitative expression of phospholipase C zeta, as an index to assess fertilization potential of a semen sample. Hum Reprod 26: 2950-6

Ito J, Parrington J, Fissore RA. (2011) PLCζ and its role as a trigger of development in vertebrates. Mol Reprod Dev 78: 846-53

Coward K, Ponting CP, Zhang N, Young C, Huang CJ, Chou CM, Kashir J, Fissore RA, Parrington J. (2011) Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish. Mol Reprod Dev 78: 48-56

Parrington J, Coward K, Gadea J. (2011) Sperm and testis mediated DNA transfer as a means of gene therapy. Syst Biol Reprod Med 57: 35-42

Galione A, Morgan AJ, Arredouani A, Davis LC, Rietdorf K, Ruas M, Parrington J. (2010) NAADP as an intracellular messenger regulating lysosomal calcium-release channels. Biochem Soc Trans 38: 1424-31

 Arredouani A, Evans AM, Ma J, Parrington J, Zhu MX, Galione A. (2010) An emerging role for NAADP-mediated Ca²⁺ signaling in the pancreatic β-cell. Islets 2: 323-30

Heytens E, Schmitt-John T, Moser JM, Jensen NM, Soleimani R, Young C, Coward K, Parrington J, De Sutter P. (2010) Reduced fertilization after ICSI and abnormal phospholipase C zeta presence in spermatozoa from the wobbler mouse. Reprod Biomed Online 21: 742-9

 Pitt SJ, Funnell TM, Sitsapesan M, Venturi E, Rietdorf K, Ruas M, Ganesan A, Gosain R, Churchill GC, Zhu MX, Parrington J, Galione A, Sitsapesan R. (2010) TPC2 is a novel NAADP-sensitive Ca²⁺ release channel, operating as a dual sensor of luminal pH and Ca²⁺. J Biol Chem 285: 35039-46

Tugba Durlu-Kandilci N, Ruas M, Chuang KT, Brading A, Parrington J, Galione A. (2010) TPC2 proteins mediate nicotinic acid adenine dinucleotide phosphate (NAADP)- and agonist-evoked contractions of smooth muscle. J Biol Chem 285: 24925-32

Ruas M, Rietdorf K, Arredouani A, Davis LC, Lloyd-Evans E, Koegel H, Funnell TM, Morgan AJ, Ward JA, Watanabe K, Cheng X, Churchill GC, Zhu MX, Platt FM, Wessel GM, Parrington J, Galione A. (2010) Purified TPC Isoforms Form NAADP Receptors with Distinct Roles for Ca²⁺ Signaling and Endolysosomal Trafficking. Curr Biol 20(8): 703-9

 Zhu MX, Ma J, Parrington J, Galione A, Mark Evans A. (2010) TPCs: Endolysosomal channels for Ca²⁺ mobilization from acidic organelles triggered by NAADP. FEBS Lett 584(10): 1966-74

Heytens E, Parrington J, Coward K, Young C, Lambrecht S, Yoon SY, Fissore RA, Hamer R, Deane CM, Ruas M, Grasa P, Soleimani R, Cuvelier CA, Gerrisa J, Dhont M, Deforce D, Leybaert L, De Sutter P (2009) Reduced amounts and abnormal forms of phospholipase C zeta in spermatozoa from infertile men. Hum Reprod 24: 2417-28.

Calcraft PJ, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X, Tang J, Rietdorf K, Teboul L, Chuang KT, Lin P, Xiao R, Wang C, Zhu Y, Lin Y, Wyatt CN, Parrington J, Ma J, Evans AM, Galione A, Zhu MX (2009) NAADP mobilizes calcium from acidic organelles through two-pore channels. Nature 459: 596-600


Young C, Grasa P, Coward K, Davis LC, Parrington J (2009) Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction. Fertil Steril. 91: 2230-42

Davis LC, Morgan AJ, Ruas M, Wong JL, Graeff RM, Poustka AJ, Lee HC, Wessel GM, Parrington J, Galione A (2008) Ca²⁺ signaling occurs via second messenger release from intraorganelle synthesis sites. Curr Biol 18: 1612-8

Yoon SY, Jellerette T, Salicioni AM, Lee HC, Yoo MS, Coward K, Parrington J, Grow D, Cibelli JB, Visconti PE, Mager J, Fissore RA (2008) Human sperm devoid of PLC, zeta 1 fail to induce Ca²⁺ release and are unable to initiate the first step of embryo development. J Clin Invest 118:  3671-81

Grasa P, Coward K, Young C, Parrington J (2008) The pattern of localization of the putative oocyte activation factor, phospholipase Czeta, in uncapacitated, capacitated, and ionophore-treated human spermatozoa. Hum Reprod 23: 2513-22

Parrington J, Davis LC, Galione A, Wessel G (2007) Flipping the switch: how a sperm activates the egg at fertilization. Dev Dyn 236: 2027-38

Coward K, Kubota H, Parrington J. (2007) In vivo gene transfer into testis and sperm: developments and future application. Arch Androl 53: 187-97

Coward K, Owen H, Tunwell R, Swann K, Parrington J. (2007) Phospholipid binding properties and functional characterization of a sea urchin phospholipase Cdelta in urchin and mouse eggs. Biochem Biophys Res Commun 357: 964-70

Mattick P, Parrington J, Odia E, Simpson A, Collins T, Terrar D (2007) Ca²⁺ stimulated adenylyl cyclase isoform AC1 is preferentially expressed in guinea-pig sino atrial node cells and modulates the I(f) pacemaker current. J Physiol 582: 1195-20

Coward K, Hibbitt O, Kubota H, McIlhinney J, Kohri K, Parrington J. (2006) Expression of a fluorescent recombinant form of sperm protein PLCzeta in mouse pididymal sperm by in vivo gene transfer into the testis. Fertil Steril 85: 1281-9

Kubota H, Hayashi Y, Kubota Y, Umemoto Y, Coward K, Parrington J (2005) Comparison of two methods of in vivo gene transfer by electroporation. Fertility & Sterility  83 Suppl 4: 1310-8

Coward K, Ponting CP, Chang, HY, Hibbitt O, Savolainen P, Jones KT, Parrington J. (2005) Phospholipase C zeta, the trigger of egg activation in mammals, is present in a non-mammalian species. Reproduction 130: 157-63